1,647 research outputs found

    Detecting Search and Rescue Targets in Moving Aerial Images Using Eye-Gaze

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    Eye gaze performance is measured and characterised in the context of moving image inspection. The application domain is that of Wilderness Search and Rescue. Here, moving terrain images captured by an Unmanned Aerial Vehicle (UAV) must be inspected accurately and with minimal delay to attempt identification of a missing person or related artefacts. An exploratory pilot experiment using images of real terrain established the extent of gaze for North and East movement of the UAV at four different speeds, and also for the condition in which a sequence of static frames of the same moving image was inspected. Observation were also made of the gaze tracking behaviour initiated by a potential target. Suggestions are made about the way in which the observation and characterization of eye gaze may be harnessed to enhance the success of target detection in a search and rescue context.Accepted versio

    Analysis and calibration of absorptive images of Bose-Einstein condensate at non-zero temperatures

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    We describe the method allowing quantitative interpretation of absorptive images of mixtures of BEC and thermal atoms which reduces possible systematic errors associated with evaluation of the contribution of each fraction. By using known temperature dependence of the BEC fraction, the analysis allows precise calibration of the fitting results. The developed method is verified in two different measurements and compares well with theoretical calculations and with measurements performed by another group.Comment: 17 pages, 8 figure

    Current challenges in de novo plant genome sequencing and assembly

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    ABSTRACT: Genome sequencing is now affordable, but assembling plant genomes de novo remains challenging. We assess the state of the art of assembly and review the best practices for the community

    Wnt3a nanodisks promote ex vivo expansion of hematopoietic stem and progenitor cells

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    BACKGROUND: Wnt proteins modulate development, stem cell fate and cancer through interactions with cell surface receptors. Wnts are cysteine-rich, glycosylated, lipid modified, two domain proteins that are prone to aggregation. The culprit responsible for this behavior is a covalently bound palmitoleoyl moiety in the N-terminal domain. RESULTS: By combining murine Wnt3a with phospholipid and apolipoprotein A-I, ternary complexes termed nanodisks (ND) were generated. ND-associated Wnt3a is soluble in the absence of detergent micelles and gel filtration chromatography revealed that Wnt3a co-elutes with ND. In signaling assays, Wnt3a ND induced β-catenin stabilization in mouse fibroblasts as well as hematopoietic stem and progenitor cells (HSPC). Prolonged exposure of HSPC to Wnt3a ND stimulated proliferation and expansion of Lin(−) Sca-1(+) c-Kit(+) cells. Surprisingly, ND lacking Wnt3a contributed to Lin(−) Sca-1(+) c-Kit(+) cell expansion, an effect that was not mediated through β-catenin. CONCLUSIONS: The data indicate Wnt3a ND constitute a water-soluble transport vehicle capable of promoting ex vivo expansion of HSPC. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12951-016-0218-5) contains supplementary material, which is available to authorized users

    Simonsenia aveniformis sp nov (Bacillariophyceae), molecular phylogeny and systematics of the genus, and a new type of canal raphe system

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    The genus Simonsenia is reviewed and S. aveniformis described as new for science by light and electron microscopy. The new species originated from estuarine environments in southern Iberia (Atlantic coast) and was isolated into culture. In LM, Simonsenia resembles Nitzschia, with bridges (fibulae) beneath the raphe, which is marginal. It is only electron microscope (EM) examination that reveals the true structure of the raphe system, which consists of a raphe canal raised on a keel (wing), supported by rib like braces (fenestral bars) and tube-like portulae; between the portulae the keel is perforated by open windows (fenestrae). Based on the presence of portulae and a fenestrated keel, Simonsenia has been proposed to be intermediate between Bacillariaceae and Surirellaceae. However, an rbcL phylogeny revealed that Simonsenia belongs firmly in the Bacillariaceae, with which it shares a similar chloroplast arrangement, rather than in the Surirellaceae. Lack of homology between the surirelloid and simonsenioid keels is reflected in subtle differences in the morphology and ontogeny of the portulae and fenestrae. The diversity of Simonsenia has probably been underestimated, particularly in the marine environment.Polish National Science Centre in Cracow within the Maestro program [N 2012/04/A/ST10/00544]; Sciences and Technologies Foundation-FCT (Portugal) [SFRH/BD/62405/2009]info:eu-repo/semantics/publishedVersio
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